htlr9 cells Search Results


hek blue htlr9  (InvivoGen)
94
InvivoGen hek blue htlr9
CpG 2395, DNA 2395, and TNA 2395 activate Ramos and Raji cells, while only CpG 2395 induces SEAP reporter activity in HEK-Blue <t>hTLR9</t> cells. (A, B) Ramos and Raji cells were plated at a density of 2 × 105 cells in 12-well plates and stimulated with the indicated ligands at the indicated concentrations. After 72 h, cells were harvested for flow cytometric analysis of surface-expressed CD86 expressing using a PE-labeled antibody specific for CD86. A PE-labeled isotype antibody was included as a control. Data are shown relative to the untreated control for Ramos (A) and Raji (B) cells. Error bars represent the standard deviation between two replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. (C, D) HEK-Blue hTLR9 cells were plated at a density of 5 × 104 in 96-well plates and allowed to adhere overnight. Cells were then stimulated with the indicated ligands at the indicated concentrations. After 24 (A) or 72 (B) hours, supernatants were harvested and tested for SEAP activity using QUANTI-Blue reagent. Samples were assayed in triplicate. Error bars represent standard deviations for three replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. PE, phycoerythrin; SEAP, secreted alkaline phosphatase.
Hek Blue Htlr9, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek blue htlr9/product/InvivoGen
Average 94 stars, based on 1 article reviews
hek blue htlr9 - by Bioz Stars, 2026-02
94/100 stars
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htlr9 cells  (InvivoGen)
94
InvivoGen htlr9 cells
CpG 2395, DNA 2395, and TNA 2395 activate Ramos and Raji cells, while only CpG 2395 induces SEAP reporter activity in HEK-Blue <t>hTLR9</t> cells. (A, B) Ramos and Raji cells were plated at a density of 2 × 105 cells in 12-well plates and stimulated with the indicated ligands at the indicated concentrations. After 72 h, cells were harvested for flow cytometric analysis of surface-expressed CD86 expressing using a PE-labeled antibody specific for CD86. A PE-labeled isotype antibody was included as a control. Data are shown relative to the untreated control for Ramos (A) and Raji (B) cells. Error bars represent the standard deviation between two replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. (C, D) HEK-Blue hTLR9 cells were plated at a density of 5 × 104 in 96-well plates and allowed to adhere overnight. Cells were then stimulated with the indicated ligands at the indicated concentrations. After 24 (A) or 72 (B) hours, supernatants were harvested and tested for SEAP activity using QUANTI-Blue reagent. Samples were assayed in triplicate. Error bars represent standard deviations for three replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. PE, phycoerythrin; SEAP, secreted alkaline phosphatase.
Htlr9 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/htlr9 cells/product/InvivoGen
Average 94 stars, based on 1 article reviews
htlr9 cells - by Bioz Stars, 2026-02
94/100 stars
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hekbluetm-htlr9 cells  (Vivogen Biotechnology Inc)
90
Vivogen Biotechnology Inc hekbluetm-htlr9 cells
CpG 2395, DNA 2395, and TNA 2395 activate Ramos and Raji cells, while only CpG 2395 induces SEAP reporter activity in HEK-Blue <t>hTLR9</t> cells. (A, B) Ramos and Raji cells were plated at a density of 2 × 105 cells in 12-well plates and stimulated with the indicated ligands at the indicated concentrations. After 72 h, cells were harvested for flow cytometric analysis of surface-expressed CD86 expressing using a PE-labeled antibody specific for CD86. A PE-labeled isotype antibody was included as a control. Data are shown relative to the untreated control for Ramos (A) and Raji (B) cells. Error bars represent the standard deviation between two replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. (C, D) HEK-Blue hTLR9 cells were plated at a density of 5 × 104 in 96-well plates and allowed to adhere overnight. Cells were then stimulated with the indicated ligands at the indicated concentrations. After 24 (A) or 72 (B) hours, supernatants were harvested and tested for SEAP activity using QUANTI-Blue reagent. Samples were assayed in triplicate. Error bars represent standard deviations for three replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. PE, phycoerythrin; SEAP, secreted alkaline phosphatase.
Hekbluetm Htlr9 Cells, supplied by Vivogen Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hekbluetm-htlr9 cells/product/Vivogen Biotechnology Inc
Average 90 stars, based on 1 article reviews
hekbluetm-htlr9 cells - by Bioz Stars, 2026-02
90/100 stars
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thp1-dual htlr9 cells  (InvivoGen)
96
InvivoGen thp1-dual htlr9 cells
CpG 2395, DNA 2395, and TNA 2395 activate Ramos and Raji cells, while only CpG 2395 induces SEAP reporter activity in HEK-Blue <t>hTLR9</t> cells. (A, B) Ramos and Raji cells were plated at a density of 2 × 105 cells in 12-well plates and stimulated with the indicated ligands at the indicated concentrations. After 72 h, cells were harvested for flow cytometric analysis of surface-expressed CD86 expressing using a PE-labeled antibody specific for CD86. A PE-labeled isotype antibody was included as a control. Data are shown relative to the untreated control for Ramos (A) and Raji (B) cells. Error bars represent the standard deviation between two replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. (C, D) HEK-Blue hTLR9 cells were plated at a density of 5 × 104 in 96-well plates and allowed to adhere overnight. Cells were then stimulated with the indicated ligands at the indicated concentrations. After 24 (A) or 72 (B) hours, supernatants were harvested and tested for SEAP activity using QUANTI-Blue reagent. Samples were assayed in triplicate. Error bars represent standard deviations for three replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. PE, phycoerythrin; SEAP, secreted alkaline phosphatase.
Thp1 Dual Htlr9 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thp1-dual htlr9 cells/product/InvivoGen
Average 96 stars, based on 1 article reviews
thp1-dual htlr9 cells - by Bioz Stars, 2026-02
96/100 stars
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Image Search Results


CpG 2395, DNA 2395, and TNA 2395 activate Ramos and Raji cells, while only CpG 2395 induces SEAP reporter activity in HEK-Blue hTLR9 cells. (A, B) Ramos and Raji cells were plated at a density of 2 × 105 cells in 12-well plates and stimulated with the indicated ligands at the indicated concentrations. After 72 h, cells were harvested for flow cytometric analysis of surface-expressed CD86 expressing using a PE-labeled antibody specific for CD86. A PE-labeled isotype antibody was included as a control. Data are shown relative to the untreated control for Ramos (A) and Raji (B) cells. Error bars represent the standard deviation between two replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. (C, D) HEK-Blue hTLR9 cells were plated at a density of 5 × 104 in 96-well plates and allowed to adhere overnight. Cells were then stimulated with the indicated ligands at the indicated concentrations. After 24 (A) or 72 (B) hours, supernatants were harvested and tested for SEAP activity using QUANTI-Blue reagent. Samples were assayed in triplicate. Error bars represent standard deviations for three replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. PE, phycoerythrin; SEAP, secreted alkaline phosphatase.

Journal: Nucleic Acid Therapeutics

Article Title: Activation of Innate Immune Responses by a CpG Oligonucleotide Sequence Composed Entirely of Threose Nucleic Acid

doi: 10.1089/nat.2018.0751

Figure Lengend Snippet: CpG 2395, DNA 2395, and TNA 2395 activate Ramos and Raji cells, while only CpG 2395 induces SEAP reporter activity in HEK-Blue hTLR9 cells. (A, B) Ramos and Raji cells were plated at a density of 2 × 105 cells in 12-well plates and stimulated with the indicated ligands at the indicated concentrations. After 72 h, cells were harvested for flow cytometric analysis of surface-expressed CD86 expressing using a PE-labeled antibody specific for CD86. A PE-labeled isotype antibody was included as a control. Data are shown relative to the untreated control for Ramos (A) and Raji (B) cells. Error bars represent the standard deviation between two replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. (C, D) HEK-Blue hTLR9 cells were plated at a density of 5 × 104 in 96-well plates and allowed to adhere overnight. Cells were then stimulated with the indicated ligands at the indicated concentrations. After 24 (A) or 72 (B) hours, supernatants were harvested and tested for SEAP activity using QUANTI-Blue reagent. Samples were assayed in triplicate. Error bars represent standard deviations for three replicates within a single experiment. A representative experiment is shown in each panel. Experiments were performed 3 times. PE, phycoerythrin; SEAP, secreted alkaline phosphatase.

Article Snippet: The human cell line expressing TLR9, HEK-Blue hTLR9 (Invivogen), was maintained according to the manufacturer's instructions.

Techniques: Activity Assay, Expressing, Labeling, Standard Deviation